B-galactosidase is a hydrolytic enzyme that catalyzes the breakdown of lactose, a type of sugar found in milk and other dairy products. It is produced by a variety of bacteria, including Escherichia coli, and is commonly used in molecular biology and biotechnology to aid in the digestion and modification of DNA and other biomolecules.
One of the key characteristics of b-galactosidase is its molecular weight, which is a measure of the size and mass of the enzyme. The molecular weight of b-galactosidase varies depending on the specific strain of bacteria it is derived from, but it is typically in the range of 110,000-130,000 daltons. This is relatively large compared to other enzymes, which often have molecular weights in the range of tens of thousands of daltons.
The large size of b-galactosidase is due to the fact that it is composed of multiple subunits, each of which contributes to the overall molecular weight of the enzyme. In the case of E. coli b-galactosidase, there are four subunits, each with a molecular weight of approximately 30,000 daltons. These subunits are held together by non-covalent bonds, which allow them to function as a single unit and perform their catalytic activity.
B-galactosidase has a number of important biological functions, including the digestion of lactose in the gut of mammals and the synthesis of certain biomolecules in bacteria. It has also been widely used in molecular biology and biotechnology as a tool for the analysis and modification of DNA and other biomolecules. For example, b-galactosidase can be used to cleave DNA at specific sites, allowing researchers to manipulate and study the function of specific genes.
In summary, b-galactosidase is a large enzyme with a molecular weight in the range of 110,000-130,000 daltons. It is composed of multiple subunits and plays important roles in both biology and biotechnology. Understanding the molecular structure and properties of b-galactosidase has allowed researchers to develop a range of techniques and applications that rely on this enzyme, including the analysis and manipulation of DNA and other biomolecules.
35 Greatest Speeches in History
My days of old have vanished, tone and tint. Worthy Excerpt: To assail the great and admitted evils of our political and industrial life with such crude and sweeping generalizations as to include decent men in the general condemnation means the searing of the public conscience. This one began as a response to personal abuse she received from Yoho, but became a greater statement about women and men, and the abuse women routinely receive. Great presidential speeches are made in the context of crises, challenges, and times of great peril. Mahatma Gandhi, August 8, 1942; India While the battle for freedom and democracy raged across the world, the people of India were engaged in their own fight for liberty. How much we do to Him in the person that we are serving.
Tonight I reflect on their struggle, their determination, and the strength of their vision to see what can be unburdened by what has been. Hope — Hope in the face of difficulty. Kennedy embodied the fresh optimism that had marked the post-war decade. Oh, how often you see some young fellow who boasts that he is going to "see life," meaning by that that he is going to see that part of life which it is a thousandfold better should remain unseen! And this mission would be accomplished by the end of the 1960's. He was one of the first national candidates to actually be a national candidate, hitting the speaking circuit at a time when front porch campaigns were considered the more dignified move. Franklin Delano Roosevelt, December 8, 1941; Washington, D.
12 Greatest Political Speeches in American History
Be strong and be kind. William Wilberforce, May 12, 1789; House of Commons, London When William Wilberforce, a member of the British Parliament, converted to Christianity, he began to earnestly seek to reform the evils he found within himself and the world around him. I trust, however, that mine will not be so considered. My people, some of them, have run away to the hills, and have no blankets, no food. If we were base enough to desire it, it is now too late to retire from the contest. Racism as experienced now. They didn't need thousands and thousands of words to make their point, just a few well-chosen ones given by a dynamic speaker.
It was a sad day when we finally lost John this year, and David did a brilliant job. Our greatest primary task is to put people to work. It feels largely unrehearsed, a rolling, ad lib rumination on the Black Lives Matter protests, the issues at the heart, the looting that followed, and the contract of society that is failing black people. No man has a right to shirk his political duties under whatever plea of pleasure or business; and while such shirking may be pardoned in those of small cleans it is entirely unpardonable in those among whom it is most common--in the people whose circumstances give them freedom in the struggle for life. The secret of his charisma was his sincere conviction about the ideals of the Marxist communism. At this critical moment, Churchill gave his third and final speech during the Battle of France, once again imparting words meant to bring hope in this dark hour. Our brethren are already in the field! Pathos is used for emotional appeals to gain audience acceptance.
Worthy Excerpt: It is peculiarly incumbent upon you who have strength to set a right example to others. But yet this, which ought to have been done long since, I have good reason for not doing as yet; I will put you to death, then, when there shall be not one person possible to be found so wicked, so abandoned, so like yourself, as not to allow that it has been rightly done. In this speech, he exhorts young men to pursue virtuous manliness. But, My Lord, if it needs be, it is an ideal for which I am prepared to die. I say it is to wage war by land, sea, and air.
Top 10 Famous Persuasive Speeches that Rocked the World
I am defending you. Theodore Roosevelt, April 14, 1906; Washington, D. Because I am a woman, because I am black, because I am myself, a black woman warrior poet doing my work, come to ask you, are you doing yours? Harris wore a white suit to honor the suffragettes who fought for the right for women to vote. I have been in ballparks for seventeen years and have never received anything but kindness and encouragement from you fans. Ronald Reagan Ronald Regan was a Hollywood actor and also an American politician.
The best speeches of 2020 as ranked by the Speakola website — Speakola
We want to hear new ideas and new solutions and new answers. Not by sowing hatred and division, but by spreading love and truth. Douglass helped engineer the underground railroad and strived to teach others about his experience. That was my old man who told me that. Eventually, Socrates was arrested and put on trial for corrupting the youth, not believing the gods, and creating new deities. It is a struggle for the right to live. On July 4, 1939, at the Yankee Stadium, Gehrig stood in front of a large crowd and spoke of his mandatory retirement after 2,130 consecutive baseball games and at the age of 36.
I will make those who stay the envy of those who return. She has a vast Empire behind her. Read the full text 15. Today, once again, many will feel a painful sense of separation from their loved ones. Second, we will provide all that our brave men require to do the job that must be done. This war is a worldwide war. She was recently one of the only 20 Singaporeans to be honoured on the Forbes30Under30 Asia list.